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You should first analyse the data you are provided with to determine the amount of IFNg and IL-4 produced by each of the four experimental samples (A-D). a) You should plot the standard curve of the two ELISA experiments on a graph using an appropriate analysis package (eg. Sigmaplot, etc) – ensure graphs are presented and labelled appropriately. b) You should present the results of your analysis of the experimental samples in a separate graph. c) You should describe your results (500 words maximum). Remember to include a description of what your sample and treatment groups did in comparison to controls. You should comment on what the sensitivity of the two ELISA experiments was and how significant, reliable and reproducible your results are. d) You should then give a brief discussion and conclusion for your findings (500 words maximum). Why were these two cytokines measured? What can they tell us about how the body fights the infection? Why might treatment with the anti-IL-10 agent effect the production of IFNg and IL-4 by T cells and why might this effect the health of the mice. Finally, can you find any research literature that supports these conclusions in human Leismaniasis infection?
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